Human VEGF-165, Human Cell-Expressed (HCE)

Product Features: 
  • Authentic glycosylation
  • Animal-component-free, protein-free production
  • High bioactivity and stability

Ordering

Catalog No. Pack Size Price Quantity
11644-2

10 μg

$272.00
11644-3

100 μg

$1,842.00
11644-4

1000 μg

$6,250.00

Specifications

Formulation Lyophilized in PBS
Molecular Weight 45 kDa, disulfide-linked homodimer, glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically 2 to 10 ng/ml
Purity > 95% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

11644 Purity Confirmation on SDS-PAGE
Background: 

Recombinant human VEGF165, expressed in engineered human cells, belongs to the PDGF/VEGF growth factor family. VEGF165 is a 45 kDa homodimeric disulfide-linked glycoprotein. Many cell types secrete VEGF and it is a potent angiogenic factor and mitogen that stimulates proliferation, migration and formation of endothelial cells. VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system. VEGF is induced by hypoxia, oncogene mutations and cytokines such as IL-1, IL-8 and TNF-α.

Citations

Human VEGF165 Activity Assay

11644 Activity Assay

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