Human Thrombopoietin (TPO), Human Cell-Expressed (HCE)

Product Features: 
  • Authentic human glycosylation
  • Animal-component-free, protein-free production
  • High bioactivity and stability

Ordering

Catalog No. Pack Size Price Quantity
11615-2

10 μg

$304.00
11615-3

100 μg

$2,378.00
11615-4

1000 μg

$4,252.00

Specifications

Formulation Lyophilized from a 0.2 μm filtered solution of PBS
Molecular Weight 80 to 85 kDa, monomer, glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically 0.5 to 5 ng/ml
Purity > 90% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

11615 Purity Confirmation on SDS-PAGE
Background: 

Recombinant human thrombopoietin, expressed in engineered human cells, is primarily produced in the liver and regulates the formation of megakaryocytes and platelets. C-terminal domain glycosylation is thought to be important for the secretion of TPO from cells and for survival of TPO in the circulation.

Citations

Human TPO Activity Assay

11615 Activity Assay

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