Human PDGF-AA, Human Cell-Expressed (HCE)

Product Features: 
  • Authentic human glycosylation
  • Animal-component-free, protein-free production
  • Purity > 95%

Ordering

Catalog No. Pack Size Price Quantity
11645-3

100 μg

$1,243.00
11645-4

1000 μg

$3,657.00
11645-2

10 μg

$272.00

Specifications

Formulation Lyophilized in 10 mM HOAc
Molecular Weight 35, 40 and 45 kDa, disulfide-linked homodimer, glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically 200 to 1000 ng/ml
Purity > 95% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

11645 Purity Confirmation on SDS-PAGE
Background: 

Recombinant human PDGF-AA, expressed in engineered human cells, belongs to the PDGF/VEGF protein family. PDGF-AA is expressed as a mixture of pro-form, pro-mature and mature dimers with an apparent molecular mass of 35, 40 and 45 kDa disulfide linked homodimeric glycoproteins. PDGF-AA is a growth factor for connective tissue and glial cells. Functional PDGF-AA plays a role in embryonic development, angiogenesis, cell proliferation and cell migration.

Citations

Human PDGF-AA Activity Assay

11645 Activity Assay

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