Human IL-27, Human Cell-Expressed (HCE)

Product Features: 
  • Authentic human glycosylation and native human post-translational modifications
  • Tag- and link-free
  • High bioactivity and stability

Ordering

Catalog No. Pack Size Price Quantity
11613-2

10 μg

$350.00
11613-3

100 μg

$2,136.00
11613-4

1000 μg

$8,649.00

Specifications

Formulation Lyophilized from a 0.2 μm filtered solution of PBS
Molecular Weight 25 and 30 kDa, heterodimer, glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically 4 to 12 ng/ml(IC50)
Purity > 90% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

11613 Purity Confirmation on SDS-PAGE
Background: 

Recombinant human interleukin 27 (IL-27), expressed in engineered human cells without an artificial linker or tag, is a non-covalently bonded heterodimer cytokine composed of IL-27p28 (25 kDa) and glycosylated EB13 (30 kDa). IL-27 plays an important function in regulating the activity of T-lymphocytes through the cytokine receptor WSX-I/TCCR. IL-27 is also reported to demonstrate antiviral activity with inhibitory properties on HIV-1 replication via mechanism similar to that of IFN-alpha. Similarly, IL-27 demonstrates antiviral activity on HCV replication in Huh7.5 cell line. IL-27 induces STAT-1 and -3 in the cell line.

Citations

Human IL-27 Activity Assay

11613 Activity Assay

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