Human IL-12, Human Cell-Expressed (HCE)

Product Features: 
  • Authentic human glycosylation
  • Animal-component-free, protein-free production
  • High bioactivity and stability

Ordering

Catalog No. Pack Size Price Quantity
11609-2

10 μg

$263.00
11609-3

100 μg

$2,026.00
11609-4

1000 μg

$7,140.00

Specifications

Formulation Lyophilized inPBS
Molecular Weight 57 kDa, disulfide-linked heterodimer, glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically 0.1 to 0.5 ng/ml
Purity 95% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

11609 Purity Confirmation on SDS-PAGE
Background: 

Recombinant human interleukin 12 (IL-12), expressed in engineered human cells, is a heterodimeric glycoprotein with subunits p35 and p40 cysteine-linked by a disulfide bond. The p40 dimer can bind to IL-12 receptor and antagonizes IL-12. Most cell types express the p35 chain, while the p40 chain is restricted to dendritic cells, B-cells and cells of macrophage/monocytes lineage. IL-12 is produced by macrophages and B-lymphocytes and is induced by both innate and adaptive immune responses. IL-12 may induce T-cell proliferation towards the Th1 phenotype.

Citations

Human IL-12 Activity Assay

11609 Activity Assay

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