Human Hepatocyte Growth Factor (HGF), Human Cell-Expressed (HCE)

Product Features: 
  • Authentic human glycosylation and native human post-translational modifications
  • Animal-component-free, protein-free production
  • High bioactivity and stability

Ordering

Catalog No. Pack Size Price Quantity
11618-2

10 μg

$317.00
11618-3

100 μg

$2,378.00
11618-4

1000 μg

$5,343.00

Specifications

Formulation Lyophilized from a 0.2 μm filtered solution of PBS
Molecular Weight 70 kDa, single chain, glycosylated
Source Human Cell Expressed (HCE) in HEK-293 cells in the absence of animal-derived products
EC50 Typically 10 to 50 ng/ml
Purity 95% (SDS-PAGE stained with Coomassie blue)
Endotoxin level < 1 EU/μg
Storage Lyophilized products are stable for one year at -20°C to -80°C from date of receipt. Upon reconstitution, the cytokine can be stored at 4°C for short term only (< 1 week), or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles. Refreezing should be performed on dry ice or in a dry ice/alcohol bath.

Tech Info / Data

11618 Purity Confirmation on SDS-PAGE
Background: 

Recombinant human hepatocyte growth factor (HGF), expressed in engineered human cells, is a glycosylated monomer. HGF synthesis is initiated by injuries affecting liver tissues such as hepatitis; and is a mitogen for hepatocytes in primary culture and for kidney epithelial cells and may lead to the formation of tubular structures in growing epithelial cells. HGF synchronizes its activity with GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and stimulates the growth of human melanoma cells.

Citations

Human HGF Activity Assay

11618 Activity Assay

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